ζ probe membrane Search Results


ζ probe membrane  (Bio-Rad)
96
Bio-Rad ζ probe membrane
ζ Probe Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc00025299-71-14-17?v=Bio-Rad
Average 96 stars, based on 1 article reviews
ζ probe membrane - by Bioz Stars, 2026-08
96/100 stars
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mouse monoclonal anti pkcζ antibody  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology mouse monoclonal anti pkcζ antibody
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Mouse Monoclonal Anti Pkcζ Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc05482199-32-7-11?v=Santa+Cruz+Biotechnology
Average 94 stars, based on 1 article reviews
mouse monoclonal anti pkcζ antibody - by Bioz Stars, 2026-08
94/100 stars
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ζ probe gt nylon membrane  (Bio-Rad)
96
Bio-Rad ζ probe gt nylon membrane
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
ζ Probe Gt Nylon Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc03192579-340-8-12?v=Bio-Rad
Average 96 stars, based on 1 article reviews
ζ probe gt nylon membrane - by Bioz Stars, 2026-08
96/100 stars
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nylon membrane  (Bio-Rad)
96
Bio-Rad nylon membrane
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Nylon Membrane, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc06052020__41467_2018_5208_MOESM1_ESM-218-9-11?v=Bio-Rad
Average 96 stars, based on 1 article reviews
nylon membrane - by Bioz Stars, 2026-08
96/100 stars
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membrane probes  (AnaSpec)
90
AnaSpec membrane probes
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Membrane Probes, supplied by AnaSpec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pm39939319-225-0-5?v=AnaSpec
Average 90 stars, based on 1 article reviews
membrane probes - by Bioz Stars, 2026-08
90/100 stars
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cma12 1-mm membrane probe  (CMA Microdialysis)
90
CMA Microdialysis cma12 1-mm membrane probe
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Cma12 1 Mm Membrane Probe, supplied by CMA Microdialysis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pm37997163-297-22-24?v=CMA+Microdialysis
Average 90 stars, based on 1 article reviews
cma12 1-mm membrane probe - by Bioz Stars, 2026-08
90/100 stars
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microdialysis probe membranes  (CMA Microdialysis)
90
CMA Microdialysis microdialysis probe membranes
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Microdialysis Probe Membranes, supplied by CMA Microdialysis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/10__1523_slash_jneurosci__22___13___05597__2002-65-1-19?v=CMA+Microdialysis
Average 90 stars, based on 1 article reviews
microdialysis probe membranes - by Bioz Stars, 2026-08
90/100 stars
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pc-membrane probes  (CMA Microdialysis)
90
CMA Microdialysis pc-membrane probes
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Pc Membrane Probes, supplied by CMA Microdialysis, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc03547652-494-2-11?v=CMA+Microdialysis
Average 90 stars, based on 1 article reviews
pc-membrane probes - by Bioz Stars, 2026-08
90/100 stars
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membrane probe  (Vaisala Inc)
90
Vaisala Inc membrane probe
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Membrane Probe, supplied by Vaisala Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc09457335-207-8-10?v=Vaisala+Inc
Average 90 stars, based on 1 article reviews
membrane probe - by Bioz Stars, 2026-08
90/100 stars
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membranes  (Neuro Probe)
86
Neuro Probe membranes
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Membranes, supplied by Neuro Probe, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pm21785827-70-4-7?v=Neuro+Probe
Average 86 stars, based on 1 article reviews
membranes - by Bioz Stars, 2026-08
86/100 stars
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microdialysis probes  (SciPro Inc)
90
SciPro Inc microdialysis probes
Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) <t>PKCζ</t> knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.
Microdialysis Probes, supplied by SciPro Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/%CE%B6+probe+membrane/pmc02757306-131-2-6?v=SciPro+Inc
Average 90 stars, based on 1 article reviews
microdialysis probes - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) PKCζ knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.

Journal: Bioscience Reports

Article Title: The role of PKCζ in cord blood T-cell maturation towards Th1 cytokine profile and its epigenetic regulation by fish oil

doi: 10.1042/BSR20160485

Figure Lengend Snippet: Human CB T-cells were maturated to CD45RO + /RA – phenotype by culturing with addition of PHA (day 1) and IL-2 (day 3) over 7 days. ( A ) PKCζ knockdown was achieved by nucleofection of CB T-cells with specific shRNA. Nucleofection with PKCζ shRNA led to the loss of PKCζ in T-cells, as demonstrated by Western blot of three separate CB samples. ( B ) Evaluating day 8 of cultures for the level of T-cell maturation by the levels of expression of CD45RA/CD45RO. ( C , D ) T-cells developed on PKCζ-deficient background and then stimulated with PHA/PMA were examined for (C) proliferation and (D) cytokine (TNFα, IFNγ and IL-13) production. The effects of PKCζ deficiency on cytokine synthesis are expressed as % of control production where the control values were 460 ± 115.5 pg/ml, 350.5 ± 125 pg/ml and 96 ± 57 pg/ml respectively (D). Results are expressed as mean ± S.E.M. of three experiments. Statistics: *, P <0.05; **, P <0.01.

Article Snippet: Following blocking, the membrane was probed with mouse monoclonal anti-PKCζ antibody (Santa Cruz Biotechnology, Dallas, TX, U.S.A.), washed and then incubated with HRP-conjugated sheep anti-mouse antibody.

Techniques: Knockdown, shRNA, Western Blot, Expressing, Control

CB CD4 + T-cells were obtained from 70 neonates (placebo, n =34; fish oil, n =36) and ChIP assayed for histone acetylation at promoters of genes encoding PKCζ ( PRKCZ ), IL-13 ( IL13 ), T-box 21 ( TBX21 ) and IFNγ ( IFNG ). ( A ) When compared with placebo, acetylation of histone H3 in fish oil group was higher in case of PRKCZ promoter and lower in corresponding regions of IL13 and TBX21 . ( B ) Histone H4 acetylation at IL13 promoter was lower in fish oil arm of the study than in controls. Results, expressed as relative enrichment (i.e. after normalization to RPL32 ), are given as mean ± S.E.M. Statistics: *, P <0.05; **, P <0.01.

Journal: Bioscience Reports

Article Title: The role of PKCζ in cord blood T-cell maturation towards Th1 cytokine profile and its epigenetic regulation by fish oil

doi: 10.1042/BSR20160485

Figure Lengend Snippet: CB CD4 + T-cells were obtained from 70 neonates (placebo, n =34; fish oil, n =36) and ChIP assayed for histone acetylation at promoters of genes encoding PKCζ ( PRKCZ ), IL-13 ( IL13 ), T-box 21 ( TBX21 ) and IFNγ ( IFNG ). ( A ) When compared with placebo, acetylation of histone H3 in fish oil group was higher in case of PRKCZ promoter and lower in corresponding regions of IL13 and TBX21 . ( B ) Histone H4 acetylation at IL13 promoter was lower in fish oil arm of the study than in controls. Results, expressed as relative enrichment (i.e. after normalization to RPL32 ), are given as mean ± S.E.M. Statistics: *, P <0.05; **, P <0.01.

Article Snippet: Following blocking, the membrane was probed with mouse monoclonal anti-PKCζ antibody (Santa Cruz Biotechnology, Dallas, TX, U.S.A.), washed and then incubated with HRP-conjugated sheep anti-mouse antibody.

Techniques: